Immunohistochemistry Kits and Reagents
Biotium CF543 Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange fluorescent CF543 dye has excitation/emission at 541/560 nm.Also see Biotium's CF Dye Tyramide Amplification Kits.
Biocare Medical, LLC EDTA Decloaker, 5X - 500 ml
EDTA Decloaker is a heat retrieval solution, pH 8.4-8.7. Recommended for use with antibodies with low antigen expression (cyclin D1, CD1a, CD3, CD4, CD8, CD23, bcl-6, CD61, CD79a and TdT). EDTA Decloaker is a potent agent for epitope retrieval and tissues may be 5-10 times more sensitive to immunohistochemical staining. EDTA Decloaker can be used with Biocare's digital electric pressure cooker (Decloaking Chamber), a steamer, a water bath, or a microwave oven. EDTA Decloaker incorporates Assure technology, a color-coded high temperature pH indicator solution. The end-user is assured by visual inspection that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures and will help prevent the possiblity of losing pH sensitive antigens. EDTA Decloaker is non-toxic, non-flammable, odorless and sodium azide and thimerosal free.
Medchemexpress LLC Glut3 protein, human (sf9, His) | >90.0% | 50UG
Recombinant full-length human GLUT3 protein produced in Sf9 insect cells and supplied with a C-terminal 10x His tag. The protein is provided as a 0.2 μm filtered solution in HEPES buffer containing a mild detergent, is characterized by a predicted molecular weight of approximately 55.3 kDa and >90.0% purity, and is intended for research use with recommended storage at -80°C.
- Recombinant full-length human GLUT3 expressed in Sf9 insect cells.
- C-terminal 10x His tag for affinity purification and detection.
- Supplied in 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.002% w/v LMNG.
- Predicted molecular weight ~55.3 kDa and >90.0% purity by reducing SDS-PAGE.
- Available in multiple microgram quantities for assay flexibility.
- Shipped on dry ice; store at -80°C and avoid repeated freeze-thaw cycles.
Enzo Life Sciences HIGHDEF yellow IHC chromogen (HRP) (30 ml)
Unique high definition yellow IHC chromogen for HRP-activated development. HIGHDEF yellow IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridization systems. With at least 1 day working stability, this substrate/chromogen system can be used in any automation system as well as manual use. Contents: HIGHDEF yellow IHC chromogen (HRP) substrate buffer and chromogen, Empty mixing bottle. Application: IHC, ISH (in situ hybridization). Long Term Storage: +4°C
Proteintech Group Inc RFP-Booster Alexa Fluor 568
anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins
Meso Scale Discovery V-PLEX Human ICAM-1 Kit
The V-PLEX Human ICAM-1 Kit from Meso Scale Discovery is a sandwich immunoassay for measuring human ICAM-1 in serum plasma and cell culture supernatants It includes a 96-well plate with a capture antibody a detection antibody with an electrochemiluminescent label and calibrators and controls for a standard curve The assay sensitivity is 0 23 pg/mL with an upper limit of 1500 pg/mL The electrochemiluminescent method offers high sensitivity wide range and small sample volume requirements enabling accurate quantification of human ICAM-1 levels
Biocare Medical, LLC MACH 2 Double Stain 2 - 6.0 ml
The conjugated goat anti-mouse polymer horseradish peroxidase (HRP) and the conjugated goat anti-rabbit polymer alkaline phosphatase (AP) secondary antibodies react with both heavy and light chains on mouse and rabbit IgG respectively. This innovative HRP- and AP-polymerization technology provides a significant increase in staining sensitivity when compared to other conventional HRP- or AP-conjugated secondary antibodies. Avidin-biotin blocking procedures are not necessary when using the MACH 2 conjugated secondary antibodies. It is specially designed for a rapid double stain procedure. An antibody cocktail with a mouse monoclonal and a rabbit polyclonal/monoclonal must be used. The overall staining procedure can be done with 4 major steps and in less than two hours. It can be used manually or used with automated stainers.
Abcam Hydrogen Pero x ide Blocking Reagent, 60ML
Hydrogen peroxide blocking reagent is used to block endogenous peroxidase activity in IHC applications, enhancing signal detection in HRP / peroxidase-based systems. - Cited in over 30 publications - Endogenous peroxidase block – prevents non-specific background staining in IHC - HRP-based detection – ideal for use with HRP/peroxidase detection systems - Concentration – contains 0.3% hydrogen peroxide
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Recombinant rat discoidin domain receptor 1 (DDR1) protein, His-tag | >95.0% | 50 UG
Recombinant rat discoidin domain receptor 1 (DDR1) is supplied as a His-tagged protein produced in HEK293 cells, covering residues M1-T413 (accession Q6MG19). It is intended for research use in biochemical and cell-based studies involving DDR1 signaling and binding.
- Recombinant protein expressed in HEK293 for native-like mammalian post-translational modifications.
- His-tagged for facile purification and detection.
- Includes residues M1-T413 to represent the functional receptor region.
- Purity greater than 95% as determined by reducing SDS-PAGE.
- Available in small research quantities suitable for assay development.
Meso Scale Discovery V-PLEX Plus Human ICAM-1 Kit
The Meso Scale Discovery K151SUG-4 is a 4-plate assay kit for measuring glucose in biological samples It uses electrochemiluminescent detection technology for high sensitivity and wide dynamic range The assay takes 2 5 hours and requires 25 L of sample per well It can detect glucose in the range of 0 03-1000 g/mL The kit includes plates coated with glucose oxidase calibrators controls detection antibody read buffer wash buffer and plate seals It is designed for research use only
Biotium Cytokeratin 8 (KRT8)(KRT8/2115), CF568 conjugate, 0.1mg/mL
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant')
Cayman Chemical DDR1-IN-1hydrate 5mg
A selective DDR1 inhibitor (IC50 = 105 nM; EC50 = 87 nM; inhibits colorectal cancer cell lines when used in combination with the PI3K/mTOR inhibitor, GSK2126458
Biotium CD2(BH1), CF594 conjugate, 0.1mg/mL
CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abunda
Cayman Chemical p38 MAPK PhosphoThr180 Tyr18
Antigen: phosphopeptide corresponding to amino acid residues surrounding phospho-Thr180 and phospho-Tyr182 of rat p38 MAPK • Host: rabbit • Cross Reactivity: (+) human p38 MAPK • Application(s): WB • p38 MAPK is activated by both inflammatory cytokines and by stress. It is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system. Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182.
Abcam Caspase-8 (active) FITC Staining Kit
Caspase-8 (active) FITC Staining Kit
The product is subject to the following: Abcam Restricted Use Statement